'Biofilms' jinkirin warkar da rauni na EB

Mutane hudu masu DEB da kuma mutane hudu masu JEB sun shiga aikin. Ana tattara samfurori daga raunukan da ba su nuna alamar kamuwa da cuta ba kuma ba a yi musu magani ba kwanan nan. Ana adana samfuran a -80C har sai an bincika su duka tare. Za a raba sakamakon a ƙarshen wannan aikin na shekara guda.

Jagoran bincike:

Dr Josefine Hirschfeld mataimakiyar farfesa ce kuma mai ba da shawara mai girma a Jami'ar Birmingham Dental School da Asibiti. Ta kware a aikin likitan hakora tare da mayar da hankali kan cututtukan gumaka da ƙwayoyin rigakafi da ake kira neutrophils da rawar da suke takawa a cikin cututtukan ciki har da EB.

Masu bincike:

Dokta Hadeer Ibrahim mataimakiyar malami ce a fannin ilimin cututtukan fata a Jami'ar Suez Canal da ke Masar kuma an ba ta kyautar guraben karo karatu don ba da kuɗin karatun digiri na uku a EB a Jami'ar Birmingham inda ta mai da hankali kan bincikenta kan inganta rayuwar marasa lafiya tare da. EB na daban-daban subtypes.

Farfesa Iain Chapple shi ne Shugaban Bincike na Cibiyar Nazarin Kimiyyar Kiwon Lafiyar Jama'a, Shugaban Periodontology tare da Makarantar Dentistry da Mashawarci Mai Girma a cikin Maido da Dentistry tare da Birmingham Community Health Trust.

Farfesa Adrian Heagerty kwararre ne na likitan fata kuma Farfesa mai girma a fannin ilimin fata a Jami'ar Birmingham tare da sha'awar EB. Yana jagorantar ƙungiyoyin bincike da yawa kuma ya jagoranci sabis na EB na rabin-kasa a Asibitocin Jami'ar Birmingham NHS Foundation Trust.

Dokta Sarah Kuehne babbar jami'a ce a fannin ilimin halittar jiki na baka kuma shugabar kungiyar Binciken Microbiology ta Oral a Jami'ar Birmingham. Ƙungiyar bincikenta tana da alaƙa ta kusa da Cibiyar Nazarin Kwayoyin Halitta da Kamuwa (IMI) wadda Farfesa Ian Henderson ke jagoranta.

Haɗin kai:

Dokta Annika Therese Kroeger, a Makarantar Dentistry, Jami'ar Birmingham, tana da kwarewa sosai a cikin metagenomics da kwafi.

Farfesa Moritz Kebschull, a Makarantar Dentistry, ya gudanar da binciken fassarar fassarar lambar yabo wanda ke danganta fasalin asibiti da tushen kwayoyin cuta na cututtukan lokaci-lokaci da cututtukan dasa shuki kuma shi ne mai bincike a sabuwar Cibiyar Nazarin Halittar Halitta ta NIHR.

"Muna nufin tabbatar da binciken mu na farko mai ban sha'awa cewa fina-finai na biofilms suna cikin raunuka na EB na yau da kullun waɗanda suka kasa warkewa, alhali kuwa ba sa cikin ƙananan raunuka. Za mu bincika yadda na yau da kullun biofilms ke cikin raunuka na yau da kullun, ƙwayoyin cuta a ciki, da kuma ko waɗanda suka bambanta tsakanin nau'ikan EB daban-daban. Wannan yana da matukar mahimmanci saboda biofilms suna jure wa jiyya na ƙwayoyin cuta na gargajiya, kuma suna iya zama ƙwaƙƙwaran direba na gazawar raunuka na yau da kullun don warkewa.

Mahimmanci, cututtukan da ke da alaƙa da biofilm, irin su waɗanda ke cikin baki (cutar gumi) waɗanda muka yi nazari sama da shekaru 20, ana bi da su daban da cututtukan raunuka na gargajiya. Shi kansa biofilm dole ne a rushe ta jiki kuma akwai hanyoyi masu sauƙi da yawa don cimma wannan, ya danganta da abun da ke ciki. An riga an yi amfani da waɗannan hanyoyin a cikin wasu cututtuka kuma ana iya daidaita su cikin sauri don kula da rauni na EB, mai yuwuwar canza sarrafa raunin EB. Wannan zai kasance wani ɓangare na babban aikin bincike da muke gudanarwa, wanda zai share hanya don ƙarin bincike kan yadda irin waɗannan fina-finai na halitta suke hulɗa da tsarin rigakafi na majiyyaci.

A nan gaba, za mu yi amfani da matakan anti-biofilm daban-daban waɗanda ba masu cin zarafi ba ciki har da yin amfani da wakilai don wargaza matrix na biofilm da kuma amfani da hasken haske wanda zai keɓanta da nau'ikan ƙwayoyin cuta da aka gano. "

- Dr Hirschfeld

Sunan baiwa: Amfani da takamaiman dabarun nazarin kwayoyin halitta don gano ƙananan ƙwayoyin cuta waɗanda za su iya taka rawa a cikin raunin EB.

Raunin da ba a warkar da shi ba a cikin marasa lafiya na EB suna ba da gagarumar cututtuka da ƙananan ingancin rayuwa. Matsalolin sun haɗa da: buƙatar kula da rauni mai tsawo, ƙaiƙayi, zafi, ciwon daji na fata da kuma haɗuwa da yatsun kafa wanda ake kira "hannun mitten".

Mun fahimci yadda da kuma dalilin da yasa EB blisters ke samuwa, amma ba mu san dalilin da yasa wasu blisters suka kasa warkewa na tsawon watanni ko shekaru ba duk da kula da raunuka masu kyau. Mun yi imanin cewa dalilin da ya sa na iya zama kasancewar "biofilms" a kan raunin rauni.

Biofilms tarin ƙwayoyin cuta ne daban-daban waɗanda ke rayuwa a cikin matrix ɗin siriri mai ƙima, wanda ke haɗe zuwa saman kamar fata kuma galibi yana jure wa jiyya na gargajiya.

Biofilms an san su dagula warkar da raunuka da raunuka da ke ɗauke da fina-finai akai-akai ba su nuna alamun kamuwa da cuta kamar kumburi da kumburi / ja. Bugu da ƙari, biofilms ba za a iya gano ta hanyar hanyoyin swabbing raunuka na gargajiya ba, kuma kada ku amsa ga kula da raunuka na yau da kullum da kuma amfani da kwayoyin cuta. Duk da haka, raunuka tare da biofilms da aka yi nazari a wasu cututtuka ciki har da ciwon sukari, ƙonewa da raunuka na venous, na iya amsa da kyau ga matakan anti-biofilm wanda ke haifar da ingantacciyar warkarwa. Binciken mu na farko ta amfani da dabaru daban-daban na hoto ya nuna, a karon farko, kasancewar biofilms akan raunuka na yau da kullun na nau'ikan EB daban-daban.

Wannan shawarar tana nufin tabbatar da sakamakon bincikenmu ta hanyar nazarin kwayoyin halitta na waɗannan filayen halittu don bayyana nau'ikan ƙwayoyin cuta da ke akwai da kuma yiwuwar bambance-bambance tsakanin nau'ikan EB.

Ba a taɓa yin nazarin wannan ba, zai haɗa da yin amfani da sabbin dabaru kuma zai zama aikin haɗin gwiwa da yawa. Wannan zai taimaka mana haɓaka sabbin hanyoyin kwantar da hankali ta hanyar da aka fi niyya, kamar yadda za mu sami alamu masu ƙarfi game da dalilin da yasa raunuka suka kasa warkewa. Wannan zai haifar da hanyar da aka fi dacewa da keɓancewa ga jiyya, yana haifar da ingantattun dabarun kula da rauni da kuma taimakawa dacewar warkar da raunuka na yau da kullun a cikin marasa lafiya na EB.

Muna kan aiwatar da tarin samfurori. Ana tattara samfurori daga marasa lafiya tare da EB waɗanda ke da mummunan rauni / raunuka masu tsanani waɗanda ba su nuna alamun kamuwa da cuta ba kuma ba a bi da su tare da maganin rigakafi kwanan nan ba. Muna nufin tattara samfurori daga marasa lafiya 5 tare da dystrophic EB (DEB) kuma aƙalla 3 tare da junctional EB (JEB). Tarin samfurin daga wannan yanayin fata da ba kasafai ba yana da ƙalubale, kuma muna buƙatar amfani da ƙa'idodin haɗa / keɓance mu. Duk da haka, ya zuwa yanzu, mun yi nasarar daukar majinyatan DEB 4 da 4 JEB. Ana adana samfurori a cikin injin daskarewa -80C don aiki daga baya da zarar an tattara duk samfuran. (Daga rahoton ci gaba Afrilu 2024.)